Scanning over the save file, I see you have the following unique girls in your brothels:
Alena
Etna
Cecilia
KiKi
Carrera
Kos Mos
Jessica Albert
Boa Hancock
Akizuki Ritsuko
Amami Haruka
Akiyama Mio
Hong Meiling
Shameimaru Aya
Kirisame Marisa
Hakurei Reimu
Ayase Yue
Karakuri Chachamaru
Konoe Konoka
Hasegawa Chisame
Nagase Kaede
Mahora Cheerleaders
Asakura Kazumi
Tatsumiya Mana
Sakurazaki Setsuna
Miyazaki Nodoka
Kuu Fei
Yukihiro Ayaka
Evangeline A. K. Mcdowell
Narutaki Fuuka & Fumika
Sasaki Makie
Chao Lingshen
Saotome Haruna
Naba Chizuru
Chun-Li
Kooh
Cassandra Alexandra
Astaroth
Cammy White
Asakura Ryouko
Cecile Croomy
Asahina Mikuru
Ayanami Rei
Hana
Bulma
Android 18
Aisaka Taiga
According to the save, there aren't any more unique girls in your game. Did you add the others to your characters folder after you started this game? If so, then either a) your .girls/.girlsx files are missing or corrupted, b) you added the new girls to an existing .girls/.girlsx file (the game only loads a given .girls/.girlsx file once, so if you update one the changes won't appear in games you have already started), or c) the mechanism that adds new girls to existing games is still broken.
If you've added girls since you started this game, try starting a new one and see if the missing girls show up.